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产品目录

BmtI内切酶图片
产品货号:
GS2217
中文名称:
BmtI内切酶
英文名称:
BmtI
产品规格:
200U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.13.BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(Hind III-digest)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesGCTAG↑C
来源E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2
Assayed onLambda DNA(Hind III-digest)
Optimal bufferW(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
LigationsAfter 20-fold overdigestion with enzyme about 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsAsuNHI?^,BspOI,NheI^

相关搜索:BmtI内切酶BmtI限制性内切酶
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