产品货号：
GS2217
中文名称：
BmtI内切酶
英文名称：
BmtI
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.13.BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
技术规格
相关搜索：BmtI内切酶，BmtI限制性内切酶
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.13.BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(Hind III-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GCTAG↑C |
| 来源 | E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2 |
| Assayed on | Lambda DNA(Hind III-digest) |
| Optimal buffer | W(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme about 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | AsuNHI?^,BspOI,NheI^ |
相关搜索：BmtI内切酶，BmtI限制性内切酶